ASCP-MLT Exam Dumps - MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)

Caffeine benzoate solution is used to split the unconjugated bilirubin protein complex releasing the bilirubin so that it can react with diazotised sulphanilic acid. The tartrate buffer creates an alkaline solution and converts the red acid bilirubin to a green coloured compound which can be measured spectrophotometrically.

Which substance is used in the Jendrassik-Grof method to accelerate the reaction of unconjugated bilirubin with the diazo reagent?

Alkaline phosphatase, or ALP, is present in kidneys, liver, intestines, bone, and the placenta. The liver makes the largest amount of ALP. Some of the conditions associated with increased levels of ALP include: damaged liver cells, rapid bone growth (during puberty), bone diseases, or a disease that affects how much calcium is in the blood (hyperparathyroidism), and vitamin D deficiency.

Chemistry

Isoenzymes of alkaline phosphatase occur in:

P. vivax characteristically displays Schuffner's dots and often enlarged RBCs along with brownish granules. P. vivax can also have 12-24 merozoies in each cell, actually filling the entire RBC. This parasite also has very irreglar shapes often referred to as "Ameboid".

P. falciparum and P. malariae do not display Shuffner's dots, therefore could not be the correct choice.

P. ovale does display Shuffner's dots in all stages, but characteristically has about 8-12 merozoites in rosettes or irregular clusters inside the RBC. Also, P. ovale characteristically shows enlarged, ovoid RBCs with fimbriated edges.

Identify the parasite of a patient with suspected malaria who demonstrates the following findings on a blood smear:

- Enlarged RBCs, some with fine brownish granules

- > 15 parasites in some cells

- Ameboid structures

- Schuffner's dots

A patient with influenza would be placed in:

Microbiology

Matching: The detection of a distinct odor is often helpful in the presumptive identification of bacterial culture isolates. Match each of the odors listed with its corresponding bacterial species name.

1. Streptococcus anginosus (milleri)

2. Pseudomonas aeruginosa

3. Eikenella corrodens

4. Alcaligenes faecalis

The laboratory uses flammable chemicals for processing and staining patient specimens. One type of chemical used is ethanol. What is the proper way to store this chemical in the laboratory?

The best course of action when entering an isolation room is:

Any oxidase-positive organism can be excluded from the Enterobacteriaceae. The other characteristics are as a rule present in these organisms.

Which of the following is not true about members of the Enterobacteriaceae: